Journal: EMBO Molecular Medicine

Article Title: Genetic partitioning of interleukin-6 signalling in mice dissociates Stat3 from Smad3-mediated lung fibrosis

doi: 10.1002/emmm.201100604

Figure Lengend Snippet: Masson's trichrome stained sections of lung from gp130 wt (wt), Smad3 −/− (Smad3 −/− ), gp130 757F (757F) or gp130 757F ; Smad3 −/− (757F;Smad3 −/− ) mice 21 days after challenge with bleomycin or saline. Images are representative of n = 3 mice. Scale bar = 500 µm (= 100 µm insets). Percent change in collagen content in lung homogenates between saline and bleomycin treated mice of the indicated genotypes 30 days after challenge. n ≥ 3 mice. The range of collagen levels between saline and bleomycin treated mice was 4.369–26.48 mg. Data are expressed as mean percentage change ± SEM, with * p < 0.05 using Bonferroni multiple comparisons test. Masson's trichrome stained section of lungs from wt (top) or Smad3 −/− (bottom) mice 14 days after trans-nasal delivery of control (left) or Ad-mOsm virus (right). Images are representative of three mice. Scale bar = 500µm (= 100 µm insets).

Article Snippet: Antibodies directed against B220 were from Becton Dickinson Biosciences (San Jose, USA), CD3 from Serotec (Kidlington, UK), F4/80 from Abcam (Cambridge, UK), α-tubulin (Sigma–Aldrich), CD45 and cytokeratin AE1/AE3 from Dako (Glostrup, DK), phosphorylated Stat3 (Cell Signaling Technology, Danvers, USA) and β-actin, Stat3 or phosphorylated Smad3 (Santa Cruz Biotechnology).

Techniques: Staining, Saline, Control, Virus